Biochempages
Q/A
April 28, 2016
Bradford assay is a protein quantification protocol developed by Marion Mckinley Bradford in 1976. It gives an easy way to estimate the protein concentration of a solution using a standard curve generated by the use of known concentrations of a protein. First, you need to prepare …
Biochempages
Q/A
April 28, 2016
To prepare a 50 mM phosphate buffer of pH 6.8, you may use either sodium salt or potassium salt of the orthophosphoric acid. There are two components of the phosphate buffer; acid and salt (conjugate base). For sodium phosphate buffer, acid is sodium dihydrogen phosphate and …
Biochempages
Q/A
April 9, 2016
Before answering the question of where does a peptide bond form, I would like to say that protein is synthesized in the ribosome located in the cytosol. A ribosome is a protein (practically polypeptide) synthesizing apparatus where matured mRNA (after processing) comes and genetic information in …
Biochempages
Q/A
April 9, 2016
Before going to the main topic “how can a lipid be distinguished from a sugar”, you have to know what those are and what those are composed of. Lipid and sugar both are biomolecules and play important roles in their own way. However, sugar molecules are …
Biochempages
Q/A
February 16, 2016
Before I explain what maintains the secondary structure of a protein, I would like to explain about the secondary structure of a protein. I hope you know about the primary structure of the protein (the polypeptide chain). The secondary structure of a protein is a regular …
Biochempages
Q/A
February 14, 2016
Before discussing what characteristic of phospholipids is important to their function?, I would like to define what phospholipids are. Phospholipids are the most abundant type of lipids mainly found in the cell membrane and membranes of different organelles. If you look at the classification of lipids, …
Biochempages
Q/A
February 14, 2016
Before discussing what atoms make up carbohydrates?, I would like to discuss what is carbohydrates? Carbohydrates are the one of the four macromolecules of the biological system. There are four macromolecules in the biological system and these are Proteins, Carbohydrates, Lipids and Nucleic Acids. Except for …