Online phosphate buffer calculator

Biochempages June 24, 2017 No Comments

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This is a phosphate buffer calculator that I have developed for you to calculate the required amount of individual components. You can manually calculate the amounts of individual components of a phosphate buffer but that will take a lot of time. To save your time in such a lengthy calculation, I have developed this online form as a Phosphate buffer calculator. You just need to enter the necessary information in the form and you will automatically get the amounts of individual components that you need. To know more about the manual calculation of phosphate buffer components, visit this; How to prepare 100 ml of 50 mM Phosphate buffer of pH 6.8?

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Phosphate buffer is an important buffer that we use in the biochemistry laboratories. It is often used to extract and isolate proteins and enzymes that have optimal pH around 7. That is because buffering capacity of a phosphate buffer is pH 7.21 ± 1 within which most of the proteins and enzymes are more stable. For a phosphate buffer, you will need two components; dihydrogen phosphate ion and hydrogen phosphate ion. Actually, you can use either sodium phosphate or potassium phosphate buffer for the preparation of a phosphate buffer. Sometimes, researchers also use a combination of sodium and potassium phosphate for the preparation of phosphate buffer to balance the sodium and potassium ion requirements. Anyway, both work well as long as you have prepared the buffer in a right way.

Though there are many other online phosphate buffer calculators such as phosphate buffer calculator provided by Centre for Proteome Research, Liverpool, Buffers in Molecular Biology by Tripod.com  buffer calculator by Biomol.net, Phosphate buffer calculator provided by Altervista.org and work well with various conditions. However, they take some time to give you the results. You click on the calculate button and wait for a while until you see the results.