Biochempages
Q/A
April 28, 2016
To prepare a 50 mM phosphate buffer of pH 6.8, you may use either sodium salt or potassium salt of the orthophosphoric acid. There are two components of the phosphate buffer; acid and salt (conjugate base). For sodium phosphate buffer, acid is sodium dihydrogen phosphate and …
Urea and formamide are used as denaturants in Denaturing Gradient Gel Electrophoresis (DGGE). In DGGE, urea, and formamide denature the DNA fragments by disrupting the Hydrogen bonding between the nitrogenous base pairs. As shown in the figure above, urea and formamide form a Hydrogen bond with nitrogenous bases …
Biochempages
Q/A
April 9, 2016
Before answering the question of where does a peptide bond form, I would like to say that protein is synthesized in the ribosome located in the cytosol. A ribosome is a protein (practically polypeptide) synthesizing apparatus where matured mRNA (after processing) comes and genetic information in …
Biochempages
Q/A
April 9, 2016
Before going to the main topic “how can a lipid be distinguished from a sugar”, you have to know what those are and what those are composed of. Lipid and sugar both are biomolecules and play important roles in their own way. However, sugar molecules are …
