How to check enzyme activity of Tryptophan aminotransferase?
BiochempagesQ/A April 29, 2016 0Tryptophan aminotransferase is an enzyme that catalyzes the transamination of L-tryptophan. It requires pyridoxal phosphate as a cofactor and an amino group acceptor alpha-ketoglutarate. Tryptophan aminotransferase catalyzes the first step of indole-3-acetic acid (an auxin) biosynthesis …Read More
How to check the activity of Indole-3-Pyruvate Decarboxylase?
BiochempagesQ/A April 29, 2016 0Indole-3-pyruvate decarboxylase is an important enzyme involved in the biosynthesis of indole-3-acetic acid, a plant growth hormone. The indole-3-acetic acid biosynthetic pathway involving indole-3-pyruvate decarboxylase is called as indole-3-pyruvate pathway and indole-3-pyruvate is the key …Read More
How to check activity of Tryptophan Decarboxylase?
BiochempagesQ/A April 29, 2016 0Tryptophan decarboxylase is an enzyme that catalyzes the conversion of tryptophan to tryptamine. Tryptamine is a first reaction intermediate of indole-3-acetic acid (a type of auxin) biosynthesis. Therefore, to study the tryptamine pathway of indole-3-acetic …Read More
How does L-Carnitine deficiency affect fatty acid metabolism?
BiochempagesQ/A April 28, 2016 0L-carnitine is an amino acid derivative (but not a true amino acid because true amino acid must possess an amino group and a carboxyl group at an alpha carbon) with a tertiary amine as a …Read More
How does riboflavin initiates gel polymerization?
BiochempagesQ/A April 28, 2016 0Flavin is a common name for heterotricyclic organic compounds that are based on pteridine. A ribose molecule attached to the flavin nucleus through an amine bond to the N10 atom is called as riboflavin. Riboflavin …Read More
How to calculate pKa of phosphate buffer?
BiochempagesQ/A April 28, 2016 0The pKa of phosphate buffer can be calculated by using a mathematical expression. Phosphate buffer is most commonly used in biochemistry laboratories for the experiments involving proteins and enzymes. It maintains the pH of the …Read More
What is an Anthrone reagent and its application?
BiochempagesQ/A April 28, 2016 0Anthrone reagent is a 2 g/L solution of anthrone in concentrated H2SO4. It is most frequently used to measure the concentration of not only carbohydrates in a solution but also free hexoses and aldopentoses including sugar acids …Read More
How to prepare Salkowski reagent to quantify IAA?
BiochempagesQ/A April 28, 2016 0To quantify the indole-3-acetic acid (IAA) content of a sample solution, Salkowski reagent is most frequently used. Salkowski test for IAA quantification was originally developed by Salkowski E. in 1885. Original Salkowski reagent was formulated …Read More
What is Ehmann’s Reagent and how is it prepared?
BiochempagesQ/A April 28, 2016 0Ehmann’s reagent is a mixture of Ehrlich reagent and Salkowski reagent in a ratio of 1:3 (v/v). It is used to detect indole intermediates during the thin layer chromatography. This reagent is greatly helpful to …Read More
How to extract DNA from DGGE gel for further analysis?
BiochempagesQ/A April 28, 2016 0To do downstream DNA sequence analysis after DGGE, extraction of DNA from DGGE gel is a necessary step. DGGE or denaturing gradient gel electrophoresis is a molecular tool that is used to study the microbial …Read More
What is a buffer system and its importance?
BiochempagesQ/A April 28, 2016 0A buffer system is a solution that prevents the drastic change in the concentration of H+ ions (and pH) in the system. It is useful in the biological system because proteins and enzymes are highly susceptible to …Read More
How to perform Bradford assay for protein quantification?
BiochempagesQ/A April 28, 2016 0Bradford assay is a protein quantification protocol developed by Marion Mckinley Bradford in 1976. It gives an easy was to estimate protein concentration of a solution using a standard curve generated by the use of …