Category: Biochemical Techniques

Use of the APS and TEMED in SDS-PAGE

  APS and TEMED are the polymerization starting components basically used in the polyacrylamide gel electrophoresis.  During the polyacrylamide gel electrophoresis, acrylamide and bisacrylamide are added to form a gel matrix. Acrylamide forms polymers in which bisacrylamide creates a cross-linking.  Thus, it gives the overall gel-like matrix …