Category: Electrophoresis

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Conventional methods for the agarose gel extraction of DNA

 Agarose gel extraction can be made easy by using commercially available gel extraction kits or advanced electrophoretic apparatuses that allow the visualization of DNA bands during the electrophoresis. Conventional methods include dialysis of the sliced gel containing DNA by applying an electric current while some other methods include spin columns and Sephadex …

Use of the APS and TEMED in SDS-PAGE

 APS and TEMED are the polymerization starting components basically used in the polyacrylamide gel electrophoresis.  During the polyacrylamide gel electrophoresis, acrylamide and bisacrylamide are added to form a gel matrix. Acrylamide forms polymers in which bisacrylamide creates a cross-linking.  Thus, it gives the overall gel-like matrix with …