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This is a phosphate buffer calculator that I have developed for you to calculate the required amount of individual components. You can manually calculate the amounts of individual components of a phosphate buffer but that will take a lot of time. To save your time in such a lengthy calculation, I have developed this online form as a Phosphate buffer calculator. You just need to enter the necessary information in the form and you will automatically get the amounts of individual components that you need. To know more about the manual calculation of phosphate buffer components, visit this; How to prepare 100 ml of 50 mM Phosphate buffer of pH 6.8?
Phosphate buffer is an important buffer that we use in the biochemistry laboratories. It is often used to extract and isolate proteins and enzymes that have optimal pH around 7. That is because buffering capacity of a phosphate buffer is pH 7.21 ± 1 within which most of the proteins and enzymes are more stable. For a phosphate buffer, you will need two components; dihydrogen phosphate ion and hydrogen phosphate ion. Actually, you can use either sodium phosphate or potassium phosphate buffer for the preparation of a phosphate buffer. Sometimes, researchers also use a combination of sodium and potassium phosphate for the preparation of phosphate buffer to balance the sodium and potassium ion requirements. Anyway, both work well as long as you have prepared the buffer in a right way.
Though there are many other online phosphate buffer calculators such as phosphate buffer calculator provided by Centre for Proteome Research, Liverpool, Buffers in Molecular Biology by Tripod.com buffer calculator by Biomol.net, Phosphate buffer calculator provided by Altervista.org and work well with various conditions. However, they take some time to give you the results. You click on the calculate button and wait for a while until you see the results.
Considering these facts that I had observed on these other calculators, I have developed this online phosphate buffer calculator. I bet you, you will like it more than any other calculators you have seen on other websites. It is so simple in the sense that, as soon as you finish entering the data, you will get your result. You don’t need to click on calculate button or show result button or anything else. It’s real-time and faster than other calculates.
All you need is the amounts of individual components of the phosphate buffer. As soon as you get these data here (expressed in gram), you can proceed with preparation. Weigh the required amount of each component and put in a volumetric flask. Add some distilled water and dissolve all the solutes. Once the solution is clear, add more distilled water to make the desired volume and mix it again. Now, you are done making a phosphate buffer of a specific concentration and pH. Thank you for using our phosphate buffer calculator.