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Here, I have described some of the recently developed portable agarose gel electrophoresis systems with the built-in safe imager. The built-in imager or illuminator helps us visualize the DNA migration in real-time while doing electrophoresis. These setups have been designed in such a way that removes the fear of UV-based DNA damage and UV exposure. They give DNA products with an improved cloning efficiency without the necessary of further purification.
1. The MUPIDTM-One LED Illuminator
The MUPIDTM-One LED Illuminator is an electrophoresis system. It has built-in transilluminator that can be used to visualize the DNA migration during the electrophoresis. It allows the detection of DNA fragments without the need for the UV transilluminator. This is because it has built-in blue LED light illuminator that provides you real-time monitoring of the DNA migration. The orange colored filter /cover allows your safety during the gel viewing and you do not need to wear the goggles.
The blue LED light illuminator emits the light of 470 nm that illuminates the DNA bands in the gel. However, the gel should be pre-cast stained with Midoiri Green or SYBR dyes compatible with blue illuminators. Since it doesn’t emit the short-wave UV lights, the DNA samples are safe for downstream applications. However, MUPTM-One LED Illuminator is compatible only with MUPLDTM-One gel electrophoresis power system and comes with gel casting set, gel chamber, power system, combs, gel trays (both small and large)
It can easily be used to purify the DNA fragments while performing the electrophoresis. To do so, there must be two well system; sample well and collection well at a fixed distance below the sample well. Load the samples in the sample wells and run the electrophoresis. When the samples reach into the collection well, you can collect the samples the sample from the collection well. To buy the setup, please visit the homepage of NIPPON Genetics EUROPE.
Features of the product;
- Size: 166 x 170 x 51 mm
- Viewing area: 150 x 60 mm
- Weight: 0.9 kg
- LED wavelength: 470 nm
- Filter: orange
- DNA sensitivity: about 2ng
2. BlueGelTM Electrophoresis System
BlueGelTM is another inexpensive, portable, handy electrophoresis system that is integrated with built-in blue LED safe imager. The setup can be used to visualize the DNA bands as they separate during the electrophoresis. It gives fast result and requires 10 times lesser reagents than the traditional one. This instrument is developed by minipcr and avoids the use of any other gel documentation system and transilluminators that use UV light.
It operates at 48V and requires no use of the Ethidium bromide (the most mutagenic, unsafe DNA staining dye). The system is built-in with blue LED light transilluminator (safe imager) that illuminates the DNA bands in the gel. The gel should be pre-cast stained with GreenViewTM plus DNA staining dye or any other dyes compatible with the blue illuminator. It has a two-buttons operation; one is for the run and another one is for the DNA visualization.
Since it doesn’t require the use of the UV light the electrophoresis products can directly be used for downstream applications. To take a picture of the gel, you can simply use your cell phone. For the user guide, watch this video on YouTube. If you are interested in buying this product, please visit the product website, minipcr. It costs 350USD per setup which is an affordable price.