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Extraction of DNA from agarose gel has many downstream applications in molecular biology research work and biomedical research. For instance, we often need to sequence or clone the DNA fragments obtained either by PCR or restriction digestion. Therefore, it is necessary to have the pure DNA sample to have the better sequencing or for better transformation efficiency.
Though there are many protocols for DNA separation such as DNA purification using electrophoresis and column chromatography. Among these, DNA separation by gel electrophoresis is the most common and easier one to perform with many advantages. So, here I’m going to mention some of the methods involving DNA separation by gel electrophoresis.
DNA separation by gel electrophoresis normally involves the use of agarose gel. It’s simple, convenient and easy to purify the DNA fragments. For the extraction of DNA from agarose gel, low melting point agarose is the preferable. The concentration of the agarose gel for the gel extraction procedure ranges from 0.7 to 0.8 %.
Low melting point agarose melts at a lower temperature as compared to the standard agarose. Therefore, it preserves the integrity of the double stranded DNA as high temperature might denature the double strands. Moreover, a lower concentration of agarose provides much easier electrophoretic separation of the DNA. It is also easier to dissolve the gel in the extraction buffer for the purification purpose.